When requesting a taxi, passengers are able to opt in if they want a self-driving car. Similar to the Pittsburgh vehicles, the cars aren't completely driverless, as they have a human counterpart present for backup.
In a blog post, the head of the company's advanced technology group, Anthony Levandowski, described self-driving vehicles as the "core of Uber's mission of reliable transportation".
He also predicted the future of ride-sharing transportation to be a mix of both human drivers and self-driving cars.
"We know that when people can get an affordable, reliable ride across town, things change for the better," he said. "Cities become safer, cleaner, more efficient and more affordable."
"Ride-sharing also helps cut into automobile-related deaths and drunk driving. It complements public transit, getting people to places that aren't well served by other forms of transportation."
Uber made a deal with Volvo in August to purchase 100 vehicles by the end of the year, and each has been kitted out with lidar (radar based on light), wireless technology and seven different cameras.
The introduction of Uber's autonomous fleet comes after the US Department of Transportation unveiled its plans to eradicate road accidents by increasing the number of self-driving vehicles.
However, the technology is still being refined. An accident report recently revealed that one of Google's self-driving vehicles had collided with a public bus in Mountain View, California – soon after it became the first non-human to be recognised as a driver in the US.
The tech giant has now spun off its self-driving car project into a separate company called Waymo.
Uber was set up by Travis Kalanick and Garrett Camp in 2009 as a black car service for 100 friends in San Francisco.
It allows users to order a taxi on demand, with payments automatically deducted from card details stored in the app.