Called Ještě Jednu, the shop has a footprint of just 29.5 square metres but accommodates a kitchen, ice cream bar, freezer and coffee corner.
Local practice Holky Rády Architekturu – meaning "girls who like architecture" in Czech – said it wanted to make the interior a "fun" environment using the building's arched openings and ceilings as a starting point.
This motif is picked up throughout the shop in the form of sweeping lighting fixtures and stainless steel sinks, where staff and customers can wash their sticky hands.
The prep kitchen is separated from the main ice cream bar using a glass partition, which reflects the shop's pendant lights and makes the space appear larger.
"People behind the glass become the alchemists who prepare the frozen delicacies," said Barbora Kudelová and Kristýna Sirováa, founders of Holky Rády Architekturu.
A calming palette of desaturated pastel colours was selected to allow the ice cream offering to stand out, while cool stainless steel surfaces reflect their surroundings.
The studio also incorporated subtle design references to the local area and to Italy – the birthplace of gelato.
These include the serving counter, which is fronted by a fluted white concrete panel that recalls both classical columns and the pillars of a 13th-century church nearby in Brno.
Similarly, the shop's stainless steel sinks nod to the water fountains that are often found in the streets of Italian towns and cities.
Other ice cream shops featured on Dezeen include Little Sky in Melbourne, which was designed to capture the "theatre of gelato", and an Instagram-friendly store in central London that features cloud-like ceilings and neon signage.
The photography is by Barbora Kudelová.